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Fig. 8 | Molecular Neurodegeneration

Fig. 8

From: SOD1 protein aggregates stimulate macropinocytosis in neurons to facilitate their propagation

Fig. 8

Other aggregated proteins are capable of stimulating plasma membrane ruffling (a) Laser scanning confocal micrographs of uptake of TDP-43, Httex146Q, α-synuclein, and α-lactalbumin aggregates into NSC-34 cells. Cells were incubated with the aggregated proteins (20 μg/mL) in the presence or absence of EIPA. Fixed and permealized cells were labelled with an ALEXA-488 conjugated to SA (green) and RedDot 2 (red). (b) ImageJ quantification of SOD1 uptake in to treated NSC-34 cells. Results shown as mean fluorescence intensity per cell, minimum 50 cells, ± SD, ** p < 0.01. (c) Flow cytometry of NSC-34 cells co-treated with protein aggregates and dextran-Alexa647. Results shown as means ± SD, n =3, *** P <0.001. (d) Field emission SEM of cells treated with protein aggregates

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