Fig. 1

Loss of parkin leads to an increase in cav-1. a Lysates prepared from WT and parkin KO MEF cells were analyzed by SDS-PAGE and Western blotting, and the band intensity of three independent experiments was quantified. b WT and parkin KO MEF cells were stained with anti-caveolin-1 (red) and observed by confocal microscopy. The intensity of three independent experiments was quantified. The mean intensity value of each experiment was acquired by measuring the intensity of at least 100 cells. Scale bar indicates 10 μm. c WT and parkin KO MEF cells were lysed in ice-cold 1 % Triton X-100 buffer and fractionated as described in ‘Methods’. The soluble and insoluble fractions were then analyzed using Western blotting. The transferrin receptor (TfR) was used as a marker for the non-lipid raft fractions. Band intensity of three independent experiments was quantified. d Parkin KO MEF cells were transfected with a plasmid for flag-parkin, and after 48 h, lysates were analyzed by SDS-PAGE and Western blotting, and the band intensity of three independent experiments was quantified. P values were determined using a Student’s t test. ** p < 0.01