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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Loss of parkin promotes lipid rafts-dependent endocytosis through accumulating caveolin-1: implications for Parkinson’s disease

Fig. 2

Parkin induces the degradation of cav-1 through the proteasome-dependent pathway. a Real-time RT-PCR was performed as described in ‘Methods’. WT and parkin KO MEF cells were incubated with with 10 μg/ml cyclohexamide (CHX) for the indicated times b 10 μM MG132 for 3.5 h c and 10 μM lactacystin for 3.5 h d Lysates were analyzed by SDS-PAGE and Western blotting. P values were determined using a two way ANOVA, **p < 0.01. e WT and parkin KO MEF cells cultured on poly-D-lysine coated glass were incubated with 10 μM MG132 for 3.5 h, stained with anti-cav-1 (red), and then observed by confocal microscopy. Blue indicates DAPI staining. Scale bar indicates 10 μm

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