Fig. 1
From: α-synuclein interacts with SOD1 and promotes its oligomerization
Detection of α-synuclein and SOD1 interaction using Gaussia luciferase (hGluc) protein-fragment complementation assay. a hGluc protein-fragment complementation principle: hGluc halves fused to the c-terminus of α-synuclein and SOD1 (S1, S2, SOD1-1, SOD1-2) or to the n-terminus of SOD1 (SOD1-3, SOD1-4) can restore luciferase activity due to protein-protein interactions. b Luciferase activity and western blot analysis (12 μg protein/lane) of H4 cells and c luciferase activity of conditioned medium 48 h post transfection (one way ANOVA, Tukey’s multiple comparison test, n = 6). d Extracellular complementation assay with H4 cell lysates containing 10,98 nM α-synuclein or SOD1. 12 h after combining the lysates, luciferase activity was measured (one way ANOVA, Tukey’s multiple comparison test, n = 3). Figures show representatively data from one experiment. Experiments have been repeated at least 2 times and showed similar results. (n.s. = not significant, * p < 0,05, ** p < 0,005, *** p < 0,0005) RLU = relative light unit