Fig. 2
From: α-synuclein interacts with SOD1 and promotes its oligomerization
α-synuclein and SOD1 binding in cellular, mouse and human samples. a α-synuclein was detected when SOD1-myc was immunoprecipitated from co-transfected H4 cells using anti-myc antibody. Input: 5 μg protein. b α-Synuclein immunoprecipitation of co-transfected H4 cells co-immunoprecipitated SOD1-myc. Input: 5 μg protein. c α-synuclein was detected when SOD1 was immunoprecipitated from whole brain lysates of BDF1 wt mouse using anti-SOD1 antibody. Input: 10 μg protein. d SOD1 immunoprecipitation of whole brain lysates of Thy1-α-synuclein tg mouse co-immunoprecipitated mouse and human α-synuclein. Input: 10 μg protein. e SOD1 immunoprecipitation of human erythrocytes using rabbit-anti-SOD1 antibody of human erythrocytes detects α-synuclein using mouse-anti-α-synuclein antibody. Input: 20 μg protein. IgG control: lysates were incubated with unspecific IgG-antibodies derived from the same species as the primary antibody. light chain = light chain of IgGs