Fig. 3

Co-localization of α-synuclein and SOD1 in wt mouse and human brain. a Representative confocal microscopy images of C57Bl/6 wt mouse brain sections co-immunostained for α-synuclein (Alexa 488, green) and SOD1 (Alexa 546, red). Nuclei were stained with DAPI. As control, single staining of α-synuclein and SOD1 was performed with respective primary antibody alone and with both fluorophores (Alexa 488, Alexa 546) conjugated secondary antibodies. b Representative microscopy images of human midbrain tissue co-immunostained for α-synuclein (Alexa 488, green) and SOD1 (Alexa 568, red). As control, sections were stained with both conjugated secondary antibodies (Alexa 488, Alexa 568) without primary antibodies