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Fig. 7 | Molecular Neurodegeneration

Fig. 7

From: α-synuclein interacts with SOD1 and promotes its oligomerization

Fig. 7

Mutual influence of α-synuclein and SOD1 on their dimerization/oligomerization. a Luciferase activity and western blot analysis (20 μg protein/lane) of H4 cell lysates and conditioned medium 48 h after transfection. SOD1-1 and SOD1-2 were expressed along with myc, wt, A30P or A53T α-synuclein. Data from 3 independent experiments were pooled after normalization to mean luciferase activity of SOD1-1 + SOD1-2 + myc (two tailed, unpaired student’s t-test, n = 33-36). b α-synuclein oligomerization in the presence of myc, wt, G85R or G93A SOD1-myc. 48 h post transfection, luciferase assay and western blot analysis with cell lysates (5 μg protein/lane) and conditioned medium. Data from 7 independent experiments were pooled after normalization to respective mean of the luciferase activity of S1 + S2 + myc (two tailed, unpaired student’s t-test, n = 83-84). (n.s. = not significant, * p < 0,05, ** p < 0,005, *** p < 0,0005, **** p < 0,0001)

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