Fig. 8
From: α-synuclein interacts with SOD1 and promotes its oligomerization
α-synuclein increases SOD1 oligomerization. a Luciferase activity and representative western blot of H4 cells (18 μg protein/lane) and b luciferase activity of conditioned medium of scrambled-shRNA stabile H4 cell line and two α-synuclein-shRNA stabile H4 cell lines (α-syn I, α-syn II) co-transfected with SOD1-1 and SOD1-2. Figures show pooled data from 4 independent experiments after normalization to respective mean luciferase activity of scrambled-shRNA cells (two tailed, unpaired student’s t-test, n = 8-11). c Luciferase activity measurement and representative western blot (5 µg protein/lane) of transfected H4 cells incubated with 7 μM recombinant α-synuclein or solvent control PBS. Data from 3 independent experiments were pooled after normalization to the respective PBS control (two tailed, unpaired student’s t-test, n = 10-12). (n.s. = not significant, * p < 0,05, *** p < 0,0005)