Beclin 1 localizes to endosomes in neurons. Representative images showing colocalization of beclin 1 with (a) Rab5-GFP, colocalization coefficient 0.49 ± 0.03, (b) Rab7-GFP, colocalization coefficient 0.50 ± 0.03 or (c) Golgin 97, colocalization coefficient 0.13 ± 0.01 in primary hippocampal neurons. Regions in the red boxes are enlarged below. Intensity traces (offset white lines) are plotted at right. Scale bars are 10 μm. Colocalization coefficients are the combined results of 2–3 independent experiments (n = 28-30 total fields/group).