Localization of ALK5 with VPS35 is beclin 1-dependent. Representative images of neurons infected with ctrl shRNA (a) or bec shRNA (b) stained with antibodies against ALK5 and VPS35. ALK5 is pseudocolored green and VPS35 is pseudocolored red. Regions in the box are enlarged below. Red arrowheads in (b) indicate ALK5 puncta that don’t colocalize with VPS35. c Quantification of the fraction of ALK5 colocalizing with VPS35 in cells infected with ctrl or bec shRNA, expressed as the colocalization coefficient. Data are combined results of three independent experiments (n = 27 fields/group). Planned comparison of colocalization coefficients in control and beclin 1 shRNA cells were analyzed by unpaired t-test, **p ≤ 0.01. Bar graphs are mean ± SEM.