Fig. 4

RAGE and PRAK mediate mTORC1 inactivation by Aβ treatment. a RAGE and DN-RAGE-overexpressing SH-SY5Y cells were treated with either DMSO or 2 μM monomeric Aβ for 6 h. b SH-SY5Y cells were transfected with plasmids expressing RAGE and then transiently re-transfected with PRAK siRNA or scrambled control siRNA. Cells were treated with either DMSO or 2 μM monomeric Aβ for 6 h. c RAGE and DN-RAGE-overexpressing SH-SY5Y cells were treated with either DMSO or 2 μM monomeric Aβ for 6 h. d SH-SY5Y cells were transfected with plasmids expressing RAGE and then transiently re-transfected with PRAK siRNA or scrambled control siRNA. Cells were treated with either DMSO or 2 μM monomeric Aβ for 6 h. Western blot was performed with the indicated antibodies. Quantification of protein level was performed by densitometric analysis. Data are mean ± SEM of 4 separate experiments. **P < 0.01