A rapid and cost-effective method for genotyping apolipoprotein E gene polymorphism

Table 2 Analysis of APOE genotypes and allele frequency in Chinese population

Cohort	No.	APOE genotypes (No.)						Accuracy (%)	Alleles (%)
		ε2/ε2	ε2/ε3	ε2/ε4	ε3/ε3	ε3/ε4	ε4/ε4		ε2	ε3	ε4
Zhongshan	399	2	60	6	278	51	2	100	8.77	83.58	7.65
Fujian	390	2	50	5	271	60	2	100	7.56	83.59	8.85
Huadong	369	3	43	5	265	52	1	100	7.32	84.69	7.99
Total	1158	7	153	16	814	163	5	100	7.90	83.94	8.16

Genomic DNA was extracted from peripheral blood samples obtained from three hospitals (399 samples from Zhongshan Hospital Affiliated to Xiamen University, 390 samples from Fujian Medical University Union Hospital, and 369 samples from Huadong Hospital Affiliated to Fudan University). The APOE genotypes and allele frequency were analyzed by both Real Time PCR and DNA sequencing. The 100 % accuracy was defined when APOE genotyping using the Real Time PCR assay showed 100 % concordance with DNA sequencing results

ISSN: 1750-1326