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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: Alzheimer’s disease-like APP processing in wild-type mice identifies synaptic defects as initial steps of disease progression

Fig. 6

AAV-APP/PS1 mice present memory impairments, 3 months after injection. C57Bl/6 J mice (all males) were injected at 8 weeks of age either with AAV-CAG-PS1M146L (AAV-PS1 mice used as an injected control group, n = 8) or AAV-CAG-APPSL + AAV-CAG-PS1M146L (AAV-APP/PS1 mice, n = 8) and behavioral analyses were performed three months later. a Open-field assay. Travelled distance during the Open-field task showing no significant difference between both groups. b Left panel: time in periphery/time in center ratio showing a change in emotional behavior when faced to a new environment in the AAV-APP/PS1 group (n = 7-8 mice per group). Bars represent means ± SEM. Statistical analysis was performed with Student’s t-test: *p < 0.05. Right panel: group occupancy plots for visualizing the areas in which the animals spent the most time during the test. c Y-maze assay. Travelled distance during the Y-Maze task showing no significant difference between both groups during the test session. d Left panel: percentage of distance in the new arm showing that AAV-APP/PS1 mice spent less time in the new arm than the other groups (p = 0.08; n = 7-8 mice per groups). Bars represent means ± SEM. Statistical analysis was performed with Student’s t-test. Right panel: group occupancy plots for visualizing the areas in which the animals spent the most time during the test. The arm circled in red is the new arm. e Morris Water Maze assay. Travelled distance during the Morris Water Maze task showing no significant difference between both groups during the five training days. f-g Probe trial performance at 4 h (f) and 72 h (g) after the last training session. TQ = target quadrant that housed the platform during the training sessions. OQ = mean of distance covered in the other three quadrants. Note that AAV-APP/PS1 mice were impaired in comparison to AAV-PS1 mice confirmed by no preference for the trained target quadrant. Bars represent means ± SEM. A two way ANOVA was used with experimental group and quadrant as main effects: *p < 0.05, **p < 0.01

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