Fig. 3
From: Oligomeric and phosphorylated alpha-synuclein as potential CSF biomarkers for Parkinson’s disease
Validation of ELISA specific for p-S129-α-syn. a Standard curves displaying the specificities and sensitivities for p-S129-α-syn (♦) and non-phosphorylated α-syn (■). b Histograms representing the optimization of the capture antibody (Syn-140) concentration and the background signal (BG). c Examination of inter-assay variability. Measurements were taken in duplicate, and the results show the mean ± standard deviation for each point, and the inter-assay variability was found < 15 %. d Assessment of α-syn recovery rate in human CSF. The recovery rates were calculated between 95 and 97 % from measured α-syn concentrations relative to spiked amounts