Fig. 10

ADAMTS-4 modulates the synthesis/release of neurotrophic factors by glial cells in vitro. a-c Quantitative RT-PCR for NGF (a) GDNF (b) and BDNF (c) expression in mouse adult cortical astrocyte cultures treated or not for 48 h with a human recombinant ADAMTS-4 (20, 100, 200 ng/ml). Values plotted are mean ± SEM. Mann–Whitney U-tests: ^* P < 0.05 compared to control, N = 4. d-e ELISA-measurements of NGF released in the media of mouse adult cortical astrocyte cultures treated or not for 48 h with a human recombinant ADAMTS-4 (d) or ADAMTS-1 (e) at different doses (20, 100, 200 ng/ml). Values plotted are mean ± SEM. Mann–Whitney U-tests: ^* P < 0.05 (ADAMTS-4) or P > 0.05 (ADAMTS-1) compared to control, N = 3. f-i Quantitative RT-PCR for ADAMTS-4 (f) NGF (g) GDNF (h) and BDNF (i) expression in mouse adult cortical astrocyte cultures transfected or not for 48 h with empty vector (mock) or silencing RNAs (siRNAs) targeting the expression of ADAMTS-4. Values plotted are mean ± SEM. Mann–Whitney U-tests: ^* P < 0.05 (ADAMTS-4, NGF, GDNF) or P > 0.05 (BDNF) compared to mock, N = 4. j ELISA-measurements of NGF released in the media of mouse adult cortical astrocyte cultures transfected or not for 48 h with mock or siRNAs targeting the expression of ADAMTS-4. Values plotted are mean ± SEM. Mann–Whitney U-tests: ^* P < 0.05 compared to control, N = 4. k-m Quantitative RT-PCR for NGF (k) GDNF (l) and BDNF (m) expression in mouse neonatal cerebral microglia cultures treated or not for 48 h with a human recombinant ADAMTS-4 (20, 100, 200 ng/ml). Values plotted are mean ± SEM. Mann–Whitney U-tests: ^* P < 0.05 compared to control, N = 4. n-q Quantitative RT-PCR for ADAMTS-4 (n) NGF (o) GDNF (p) and BDNF (q) expression in mouse neonatal cerebral microglia cultures transfected or not for 48 h with mock or siRNAs targeting the expression of ADAMTS-4. Values plotted are mean ± SEM. Mann–Whitney U-tests: ^* P < 0.05 (NGF, BDNF), ^*** P < 0.001 (ADAMTS-4, GDNF) compared to mock, N = 8