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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Direct α-synuclein promoter transactivation by the tumor suppressor p53

Fig. 1

Effects of UV irradiation and pharmacological activation of p53 on α-syn protein and mRNA levels. SH-SY5Y cells were treated with vehicle (Ct) or with etoposide (ETO, 150 μM, a and d) leptomycin (LM, 10 nM, b and e) and UV irradiation (UV, c and f) then α-syn protein (a-c) and mRNA levels (d-f) were analyzed as described in the Methods section. p53 and actin immunoreactivities are provided as read-out of p53 activation and gel loading controls respectively (a-c, upper panels). Bars represent the means ± SEM of 3 independent experiments performed in duplicates and are expressed as percentage of vehicle-treated or non-irradiated control cells. Statistical analyses were performed with GraphPad Prism software (www.graphpad.com version 4.00 for Windows, San Diego, California USA) by using homoscedastic, unpaired Student’s t-test. Significant differences are: *p < 0.05, **p < 0.01, ***p < 0.001

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