Fig. 3
From: Direct α-synuclein promoter transactivation by the tumor suppressor p53
Mouse and human endogenous p53 control α-syn transcription. Control mouse fibroblasts (MEF, p19arf-/-, black bars) or MEF devoid of p53 (p19arf-/- p53-/-, white bars) were assessed for α-syn protein (a and b), promoter transactivation (c) and mRNA levels (d) in basal conditions as described in the Methods section. α-syn protein (e, f), promoter transactivation (g) and mRNA levels (h) were analyzed in HCT116 control (HCT+/+, black bars) or p53-deficient (HCT-/-, white bars) cells as described in the Methods section. Bars in A-D represent the means ± SEM of 3-4 independent experiments performed in duplicates or triplicates and are expressed as percentage of control p19arf-/- (a-d) or HCT+/+ (e-h) cells. Actin expression is provided as a gel loading control in (a, e). Statistical analyses were performed with GraphPad Prism software by using homoscedastic, unpaired Student’s t-test. Significant differences are: **p < 0.01, and ***p < 0.001