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Fig. 5 | Molecular Neurodegeneration

Fig. 5

From: Direct α-synuclein promoter transactivation by the tumor suppressor p53

Fig. 5

Effect of mutated p53-mediated α-syn regulation ex-vivo. α-Synuclein protein (a), promoter transactivation (b) and mRNA levels (c) were analyzed after transient overexpression of empty pcDNA3.1 vector (EV, black bars) or vectors encoding for wild-type p53 (p53, white bars), R248W or R273H mutants of p53 (248 and 273, gray bars) in SH-SY5Y cells as described in the Methods section. Representative gels of p53 (flag) and actin immunoreactivities (a) are provided to illustrate transfection efficiency and control of gel loading, respectively. Bars represent the means ± SEM of 3 independent experiments performed in duplicates (a, c) or triplicates (b) and are expressed as percent of EV- transfected cells. Statistical analyses were performed with GraphPad Prism software by using One-way ANOVA analysis of variance coupled to a Newman Keuls post-hoc test. Significant differences are: **p < 0.01, and ***p < 0.001

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