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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Reactive astrocytes undergo M1 microglia/macrohpages-induced necroptosis in spinal cord injury

Fig. 4

Effects of necroptosis inhibition on astrocyte death after SCI in mice. a Combination of PI-labeling with immunohtistochemistry of GFAP in mice treated with PBS or Nec-1 at 5 days post-injury. Nec-1 significantly reduced the percent of PI-positive astrocytes. Bar = 50 μm. *P <0.05. n = 3. b Western-blotting of RIP3, MLKL and HMGB1 in spinal cord of PBS treated or Nec-1 treated mice at 5 days post-injury. Nec-1 significantly inhibited the expression of RIP3, MLKL and HMGB1, as compared to PBS control. *P <0.05. n = 3. c Combination of PI-labeling with immunohtistochemistry of GFAP in wild-type (WT) and RIP3−/− mice. RIP3 depletion significantly reduced the percent of PI-positive astrocytes. Bar = 50 μm. *P <0.05. n = 3. d Western-blotting of RIP3, MLKL and HMGB1 in spinal cord of WT and RIP3−/− mice. RIP3 mutation significantly reduced the SCI-induced expression of RIP3, MLKL and HMGB1. ***P <0.001, *P <0.05. n = 3

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