Table 1 Information for primary antibodies used
From: Reactive astrocytes undergo M1 microglia/macrohpages-induced necroptosis in spinal cord injury
Antibodies | Hosts | Dilutions | Sources |
|---|---|---|---|
Anti-Arginase1 | Goat | IHC 1:50, WB 1:200 | Santa Cruz |
Anti-Beclin1 | Mouse | IHC 1:200, WB 1:500 | Cell Signaling |
Anti-β-actin | Mouse | WB 1:50000 | Sigma |
Anti-CC1 | Mouse | IHC1:500 | Millipore |
Anti-GFAP | Mouse | IHC1:500 | Millipore |
| Â | Rabbit | IHC1:1000 | Dako |
Anti-HMGB1 | Rabbit | IHC 1:100, WB 1:500 | Proteintech |
Anti-iNOS | Mouse | IHC 1:200, WB 1:500 | BD |
Anti-Lamp2a | Rabbit | IHC1:200 | Abcam |
Anti-LC3 | Mouse | IHC1:500 | Cell Signaling |
Anti-MLKL | Rat | IHC 1:200, WB 1:500 | Millipore |
anti-pMLKL | Rabbit | IHC1:300 | Abcam |
Anti-MyD88 | Rabbit | IHC 1:50, WB 1:200 | Abcam |
Anti-NeuN | Mouse | IHC1:500 | Millipore |
Anti-OX42 | Mouse | IHC1:200 | Abcam |
Anti-RIP3 | Rabbit | IHC 1:200, WB 1:500 | Enzo |
| Â | Rabbit | WB 1:500 | Abcam |
Anti-TLR2 | Rabbit | IHC1:100, WB 1:200 | EPITOMICS |
Anti-TLR4 | Mouse | IHC 1:200, WB 1:500 | Abcam |
Anti-YFP | Goat | IHC1:600 | Rockland |