Fig. 7

a Time-lapse fluorescent pictures of four AM725 transgenic worms, immobilized in a PF127 gel matrix within the culture chambers. Scale bars = 100 μm. b Growth rate of SOD1-YFP aggregates in the body wall muscle cells of each worm, as estimated by measuring YFP expression area across each worm’s body during their immobilization in the gel matrix. c Average protein aggregate/worm area over time. For each worm and each time-point, the aggregate area is normalized by the worm area to take into account the size variability of each worm. A clear time-dependent increase of these values is observed over the period from 43 to 91 h upon loading on chip (day 1 to day 3 of worm adulthood). d Brightfield and fluorescent images of an immobilized worm (worm 1), as taken through a 63× NA 1.4 oil immersion objective 91 h upon worm loading into the device. These pictures allow mapping the aggregate morphology at high spatio-temporal resolution. e Superimposed brightfield and fluorescent images of an immobilized worm (worm 4), as taken through a 63× NA 1.4 oil immersion objective 43 and 60 h upon worm loading into the device. Arrows point at specific SOD1-YFP aggregates, which can be re-identified in subsequent images and tracked over time. Scale bars = 20 μm