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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Generation of aggregation prone N-terminally truncated amyloid β peptides by meprin β depends on the sequence specificity at the cleavage site

Fig. 3

BACE-1 activity is not influenced by meprin β. a, b Proteolytic activity of recombinant BACE-1 or recombinant meprin β was measured at pH 4.0 using a quenched fluorogenic peptide comprising the β-site sequence of APP Swedish mutant. BACE-1 activity was inhibited using a specific inhibitor (Inh IV, Calbiochem). Preincubation of BACE-1, a mixture of 50 % mature and 50 % zymogen enzyme (R&D Systems), with meprin β revealed no additional increase in BACE-1 activity (b). c As control meprin β activity was additionally measured at pH 7.5

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Molecular Neurodegeneration

ISSN: 1750-1326