Fig. 1

Schematic overview of gene expression in cortex and hippocampus of 9 month-old wild type and transgenic mice reveals that a 5 month vitamin D3 supplementation induces an extensive transcript dysregulation. a Graphical representation of the number of upregulated (dark grey) and downregulated (light grey) DEGs in either the hippocampus, cortex or combined regions in both Wt and Tg animals having been fed a vitamin D3 diet for 5 months. The number of genes specifically dysregulated in wild type mice is twice as large as in transgenic mice. b-c Venn diagrams indicating the number of overlapping (grey) and non overlapping DEGs when the two brain areas are compared in wild type and transgenic mice (b) and when the two strains are compared at the hippocampal or cortex level (c). d Venn diagram showing that, when hippocampal and cortical data are combined, vitamin D3 supplementation triggers the dysregulation of 366 genes in both strains. e List and top associated biological functions of the 366 DEGs common to both strains. Overall, 39 are associated to inflammatory response, 26 to cognition and 25 to Alzheimer’s disease. Fold change (FC) cutoff used for above analyses was -1.5> FC <1.5