Fig. 6

C1qa-deficiency mitigates loss of RGCs using two independent assays of cell counts 28 days following retinal I/R. (a) H&E stained and (c) Rbpms-immunolabeled retinal cross sections used to assess RGC loss between uninjured controls and three C1qa genetic backgrounds receiving ischemic injury. (b) Quantification of nuclei counted in the GCL determined there was a significant loss of cells (p < 0.01) in WT retinas but not in the C1qa-deficient retinas compared to control. (d) Counts of Rbpms⁺ RGCs displayed a statistically significant loss (p < 0.01) in ischemic eyes of WT mice compared to controls. No statistical differences were determined in ischemic retinas of C1qa ^+/− or C1qa ^−/− animals. However, a statistical difference (p < 0.01) was measured when C1qa ^−/− retinas were compared to WT. Mean ± SEM, n = 7 per group. **p < 0.01 compared to control and ##p < 0.01 compared to WT – I/R determined by One-way ANOVA followed by Holm-Sidak post test. Scale bars = 50 μm