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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Pioglitazone ameliorates the phenotype of a novel Parkinson’s disease mouse model by reducing neuroinflammation

Fig. 1

Cox deficiency in dopaminergic circuit of Cox10/DAT-cre mice. a Immunohistochemical images of TH staining on midbrain sections of three-week-old animals show no apparent difference in cell number between DAT-cre and Cox10/DAT-cre mice. (SN: substantia nigra, VTA: ventral tegmental area). b triple staining (TH-COX1-CytC) on midbrain sections of one-month-old control and Cox10 deficient mice. In control mice all the TH+ cells are also COX1+ and CytC+. Some TH- COX+ CytC+ cells are present in the region. These cells are still present in Cox10/DAT-cre mice sections (in white circles), while TH+ neurons are negative for COX1 (white arrows), indicating a selective deletion of Cox10 gene in dopaminergic cells. c Western blots in striatal homogenates of DAT-cre and Cox10/DAT-cre mice show no changes in TH, DAT and VDAC-porin levels (actin for normalization). d-e Complex IV enzymatic activity on striatal samples of one-month-old DAT-cre (lilac) and Cox10/DAT-cre (green) mice normalized to mg of protein d or to Citrate synthase e (n = 3/group)

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