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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Inhibition of the classical pathway of the complement cascade prevents early dendritic and synaptic degeneration in glaucoma

Fig. 1

DBA/2 J mice exhibit synapse loss early during glaucoma pathogenesis which is absent in D2.C1qa -/- retinas. a. Immunohistochemical labelling (PSD-95, red) to demonstrate synaptic integrity in 9 months old DBA/2 J retinas. All eyes shown had no significant retinal ganglion cell or axon loss. Using C1qa mutant DBA/2 J mice (D2.C1qa -/-) we explored whether synaptic pruning was present in these retinas. At 4 months D2.Gpnmb +, DBA/2 J and D2.C1qa -/- retinas show comparable synapse density (a, top row). By 9 months, there is significant synapse loss in retinas of DBA/2 J mice that is absent in retinas of either D2.Gpnmb + or D2.C1qa -/- (a, bottom two rows). b. The degree of PSD-95 labelling is shown. The region of interest is marked (white boxes in upper row). c. Rendered confocal images show PSD-95 localisation within IBA1+ microglia in the IPL of DBA/2 J mice. Inset shows a higher resolution image of a region of interest (white rectangle, d). d. Inset from C showing PSD95 engulfed by IBA1+ microglia, inset within d (white rectangle) shows PSD95 and DAPI only. Scale bar = 50 μm (a), 5 μm (c) and 1 μm (d and inset), error bars = SEM, *** = P < 0.001, GCL = ganglion cell layer, IPL = inner plexiform layer, INL = inner nuclear layer

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