Fig. 5
Protection of aging HD GMSNLNs from premature cell death. a The number of autophagosomes/lysosomes per μm2 in WT (hESM01) and HD (iPSHD22) GMSLNs after EVP4593 (1 μM) treatment for 14 h. The number of sections counted was 7-32 per bar. b Representative FC analysis of iPSHD22 MSNs stained with LysoTracker Green without treatment (green) and after 24 h incubation with EVP4593 100 nM (blue). c LoCD of WT and HD GMSLNs upon MG132-induced cell aging using 24 h 10 μM MG132 treatment. The WT value represents the mean LoCD for endo-iPS12-, IPSRG2L-, and hESM01-derived neurons, while the HD value represents the mean LoCD for iPSHD11-, iPSHD22-, and iPSHD34-derived neurons (n = 30). d EVP4593 safeguards against MG132-induced cell death in a dose-dependent manner. The WT value represents the mean ΔLoCD for endo-iPS12-, IPSRG2L-, and hESM01-derived neurons and the indicated drug, while the HD value represents the mean ΔLoCD for iPSHD11-, iPSHD22-, and iPSHD34-derived neurons and the indicated drug (n = 12). Each error bar represents the SEM