Fig. 10

SP600125 protected retinal I/R-induced reduction in size of superior colliculus. Regions of mouse brains containing SC from vehicle or SP600125 (5, 15, 30 mg/kg)- treated groups after 28 days post retinal I/R injury were collected for serial frozen sectioning and black gold staining. Bright field images were taken and cross-sectional area of superficial layers from each contralateral or ipsilateral side were measured and averaged in series of all stained sections. In vehicle treated groups, I/R injury induced significant area volume loss (~16 %) in the contralateral SC region compared to the ipsilateral region. However, SP600125 administration protected SC from I/R-induced area volume loss in contralateral hemispheres. Data represent mean ± SEM (n = 4–5). The ipsilalateral area volume in each brain was set as a 100 %. *: p < 0.05 between contralateral and ipsilateral volumes of SC by two-tailed, paired t-test