Fig. 11

SP600125 prevented I/R-induced impaired expression of synaptic markers VGLUT2 and PSD95 in the superior colliculus. Mouse brains from vehicle or SP600125 (15 mg/kg) treatment groups were collected for immunohistochemistry at 28 days post retinal I/R injury. Each brain section was incubated with primary antibody for presynaptic marker VGLUT2 or post-synaptic marker PSD95. a and c Images including both ipsilateral (Ipsil) and contralateral (Cont) superficial layers of the superior colliculus from vehicle treated (above) and SP600125 treated (below) mice. b and d Data represent mean ± SEM (n = 4 - 5). I/R injury resulted in significant fluorescence intensity loss of VGLUT2 (84.4 ± 19.0 %) and PSD95 (76.8 ± 12.6 %) in the contralateral SC after 28 days in vehicle-administered group (b and d). In contrast, 28-day administration of SP600125 prevented the loss of both synaptic markers (b and d). Total area volume of the ipsilateral SC defines 100 %. *: p < 0.05 between contralateral and ipsilateral volumes by two-tailed, paired Student’s t-test