Skip to main content
Fig. 5 | Molecular Neurodegeneration

Fig. 5

From: MicroRNA-7 targets Nod-like receptor protein 3 inflammasome to modulate neuroinflammation in the pathogenesis of Parkinson’s disease

Fig. 5

Nlrp3 is a target gene of microRNA-7. a Luciferase reporter assays confirm that Nlrp3 is a direct target gene of miR-7. Data are presented as the mean ± S.E.M from three independent experiments. b Transfection of miR-7 into BV2 cells significantly reduces NLRP3 expression but fails to affect procaspase-1 and caspase-1 expressions. c Anti-miR-7 upregulates NLRP3 expression but has no effect on caspase-1 maturation. Data are presented as the mean ± S.E.M from four independent experiments. d Transfection of miR-7 into BV2 cells markedly suppresses ATP or MSU triggerd upregulation of NLRP3 and caspase-1 as well as inhibits IL-1β production. e In contrast, miR-7 inhibitor exacerbates inflammasome activation characterized by aggravated NLRP3, caspase-1 expression and IL-1β release upon the stimulus of ATP or MSU. f Transfection of miR-7 suppresses WT or A53T α-Syn-induced upregulation of NLRP3 and caspase-1 as well as reduces IL-1β production. Data are presented as the mean ± S.E.M from four independent experiments. * p < 0.05, ** p < 0.01 vs. control or NC group, # p < 0.05, ## p < 0.01 vs. 30 nM of miR-7, anti-miR-7 treatment or corresponding NC plus ATP, MSU or α-Syn group

Back to article page