Fig. 7
Aβ42 protofibril treatment induces secondary neuronal cell death. The number of astrocytes in Aβ42 protofibril exposed cultures is significantly increased from 24 h (1.0 ± 0.1) to 24 h + 12 days (1.1 ± 0.1, P = 0.011) (a). Similar to astrocytes, the number of oligodendrocytes increases from 24 h (0.7 ± 0.3) to 24 h + 12 days (1.7 ± 1.5, P < 0.001) in Aβ42 protofibril treated cultures (b). Aβ42 protofibril treatment has no direct effect on neurons, but the neuronal cell number significantly decreases (from both 24 h, 0.9 ± 0.4, P = 0.0061 and 24 h + 6 days, 1.0 ± 0.4, P = 0.0083) in the treated cultures 12 days (0.7 ± 0.5) after Aβ removal (c). The relative number of viable cells in Aβ42 protofibril exposed cultures is normalized to viable cells in unexposed cultures. The experiments were performed in triplicates with independent cell cultures and 10 images/experiment were analyzed. Mann–Whitney U-test ***P < 0.001