Fig. 2From: Apolipoprotein E lipoprotein particles inhibit amyloid-β uptake through cell surface heparan sulphate proteoglycanApoE particles bind to heparin and cell surface HSPG. ApoE3 particles (3.4 μg) and apoE4 particles (3.4 μg) were applied to HiTrap Heparin HP columns attached to a FPLC and eluted with a linear salt gradient (0–1.0 M NaCl). Concentrations of apoE in each fraction were determined by ELISA (a). An average of n = 3 experiments are plotted in the graph. CHO-K1 or CHO-M1 cells were incubated with apoE3 (200 nM) (b) or apoE4 (200 nM) (c) for 18 h at 37 °C. Internalization of apoE was analyzed by ELISA. Data represent mean ± S.D. (n = 3). **, p < 0.01; ***, p < 0.001Back to article page