Fig. 6

ApoE particles decrease HSPG-mediated cellular uptake of Aβ in primary neurons. a Mouse primary cortical neurons were incubated with FAM-Aβ42 (500 nM) with or without apoE3 particles (200 nM) in the presence or absence of heparin (15 U/ml) for 6 h at 37 °C and analyzed by confocal microscopy. Left, middle, and right columns indicate Lysotracker, FAM-Aβ42, and merged images, respectively. b Internalization of Aβ was quantified by FACS after incubation with FAM–Aβ42 (500 nM) for 6 h. Data represent mean ± S.D. (n = 3). N.S., not significant; **, p < 0.01; ***, p < 0.001