Fig. 2

Characterization of seeding induced tau. a Soluble proteins were extracted by 1 % Triton X-100 during fixation. Representative images show that insoluble tau is recognized by phosphorylated tau antibody PHF1 in cells expressing Tau-PL-V5 and treated with Tg brain lysate, but not in other groups. The higher magnification views were shown in the bottom panel. b Representative images show that insoluble tau is thioflavin-S, total-tau, and V5 positive. The enlarged views were shown in the bottom panel. Scale bars: 100 μm