Fig. 3

Validation of seeding based cell assay under different conditions. a Western blots show decreased insoluble tau in cells treated with different concentrations of trehalose. b Quantitative analyses of (a). c Western blots show increased insoluble tau in cells treated with different concentrations of bafilomycin A1. d Quantitative analyses of (c). e Western blots show decreased insoluble tau in cells transfected with TFEB overexpression plasmid. f Quantitative analyses of (e). g Western blots show decreased insoluble tau in cells transfected with PTEN overexpression plasmid. h Quantitative analyses of (g). i Western blots show increased insoluble tau in cells transfected with Beclin1 siRNA. j Quantitative analyses of (i). k Western blots show increased insoluble tau in cells transfected with Atg5 siRNA. l Quantitative analyses of (k). Graphical data are expressed as mean ± SEM (***P < 0.001, **P < 0.01, *P < 0.05, t-test)