Fig. 4

Establishment of image based screening platform. a Experimental scheme for the image based screening platform. Seeding assay was performed in 96-well plate and subjected to thioflavin-S staining. Images were captured by EVOS fluorescent microscopy. b Representative images show thioflavin-S positive signal only in cells expressing Tau-PL-V5 and treated with Tg brain lysate, but not in other groups. c Representative images show thioflavin-S positive staining signal only in cells expressing Tau-PL-V5 and treated with PFFs, but not in other groups. d Quantitative analyses of (b). e Quantitative analyses of (c). Graphical data are expressed as mean ± SEM (***P < 0.001, t-test). Scale bars: 100 μm