Fig. 5

Validation of image based screening platform. a Representative images show decreased thioflavin-S signal in cells treated with trehalose and increased thioflavin-S signal in cells treated with bafilomycin A1. b Quantitative analyses of (a). c Representative images show decreased thioflavin-S signal in cells overexpressing TFEB or PTEN. d Quantitative analyses of (c). e Representative images show increased thioflavin-S signal in cells with knockdown of Beclin1 or Atg5. f Quantitative analyses of (e). Graphical data are expressed as mean ± SEM (***P < 0.001, **P < 0.01, *P < 0.05, t-test). Scale bars: 100 μm