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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Ex vivo imaging of active caspase 3 by a FRET-based molecular probe demonstrates the cellular dynamics and localization of the protease in cerebellar granule cells and its regulation by the apoptosis-inhibiting protein survivin

Fig. 3

Exemplificative images of the different morphologies of pSCAT3-DEVD and pHcRed1-C1-survivin double-transfected cells. a-d A CGC with the typical vertical bipolar stage morphology in the IGL. The arrow-heads indicate the axon and its branching into the parallel fiber. e-h A presumptive Golgi cell in the IGL can be identified as being larger and displaying a multipolar morphology. i-l Two CGCs in the IGL display the typical features of early apoptosis: retraction of processes and a tendency to cell shrinkage. Note that to better show the distribution of ECFP and Venus as well as the cell morphology, images are taken at different laser excitation powers. Abbreviations: CGC = cerebellar granule cell; ECFP = enhanced cyan fluorescent protein; IGL = internal granular layer of forming cerebellar cortex; Surv = survivin. Scale bars: 10 μm

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Molecular Neurodegeneration

ISSN: 1750-1326