Fig. 6
From: Early-life stress leads to impaired spatial learning and memory in middle-aged ApoE4-TR mice
Decreased expression of Reelin in the prefrontal cortex of 12-month-old ApoE4-TR mice that underwent early-life CUMS intervention. a Anti-Reelin immunohistochemical staining in the prefrontal cortex of ApoE-TR mice at 3 months and 12 months. The anti-Reelin-positive neurons were shown as dark brown dots or claws. b The quantified positively-stained Reelin in the prefrontal cortex. N = 5 per group, expressed as mean ± SEM. &&& p < 0.001, ### p < 0.001, ***p < 0.001. Scale bars are 200 um. c The expression of Reelin mRNA analyzed by real time RT-PCR in the prefrontal cortex. N = 3 per group, expressed as mean ± SEM. one-factor ANOVA of statistical methods was used. & p < 0.05, # p < 0.05, *p < 0.05