Fig. 8
From: Early-life stress leads to impaired spatial learning and memory in middle-aged ApoE4-TR mice
Decreased the levels of Glutamatergic neurons in the prefrontal cortex and the DG of the hippocampus in the 12-month-old ApoE4-TR mice that underwent early-life CUMS intervention. Immunofluorescent staining of the presynaptic gutamatergic transporter VGluT1 in the prefrontal cortex and the DG of the hippocampus in the ApoE-TR mice. Representative images (X20 magnification) are presented on the left. Quantification of the results (the mean intensity) was performed by computerized image analysis is shown on the right. N = 6 per group, expressed as mean ± SEM., one-factor ANOVA was adopt to analysis the data. &&& p < 0.001, # p < 0.05, *** p < 0.001, ** p < 0.01. Scale bars are 100 um