Fig. 3
From: Enrichment of extracellular vesicles from tissues of the central nervous system by PROSPR
Characterization of PROSPR-CNS-EVs by dedicated flow cytometry (dFC). Microparticle gates for EV size distribution analysis were set after the testing of commercial latex beads from three standard EV sizes. a Gate determination of 100 nm beads. b Gate determination of 300 nm beads. c Gate determination of 500 nm beads. d dFC measurement of PE signal from phospholipid-specific stained EVs. The gate for phospholipid-stained vesicles (R8) shows that 83.32 ± 0.65 % of the total material in PROSPR-CNS-EVs was positively stained. e Size distribution of EVs by gated analysis of stained particles from PROSPR-CNS-EVs. Lower exosomal range (≤ 100 nm) represented 74.65 ± 2.68 % of total identified microparticles. Higher exosomal range (≤ 300 nm) represented the 8.51 ± 1.11 % of total identified microparticles. Lower microvesicle range (≤ 500 nm) represented the 3.49 ± 0.17 % of total identified microparticles. Higher microvesicle range (> 500 nm) represented the 13.34 ± 1.43 % of total identified microparticles