Fig. 2

Expression of human K274/281Q tau in transgenic mice destabilizes the AIS cytoskeleton. a, b Levels of expression of human tau in the cortex of tauWT and tauKQ mice. n = 7-8 mice/group. ***p < 0.001, one-way ANOVA Bonferonni post-hoc analyses. c Representative images of βIV-spectrin and AnkG immunostaining in the somatosensory cortex of 2 month-old nontransgenic and tauKQ^high mice. Scale bars, 20 μm. d-f Representative images and quantification of the intensity and length of βIV-spectrin and AnkG immunostaining in the somatosensory cortex of 2 month-old non-transgenic and tauKQ^high mice. n = 119–132 cells from 6 mice/group. *p < 0.05, **p < 0.01, mixed-model linear regression analyses. Scale bars, 5 μm. g Representative images of βIV-spectrin and AnkG immunostaining in the somatosensory cortex of 10-12 month-old tauWT and tauKQ mice. Scale bars, 20 μm. h-j Representative images and quantification of intensity and length of βIV-spectrin and AnkG immunostaining in the somatosensory cortex of 10-12 month-old tauWT and tauKQ mice. n = 109-169 cells from 8 mice/group. *p < 0.05, **p < 0.01, mixed model linear regression analyses. Scale bars, 5 μm. Values are mean ± SEM