Fig. 3

K274/281Q tau increases MT dynamics in primary neurons. a–d Measuring MT dynamics with GFP-EB3 in rat primary neurons. a Representative images of HeLa cells co-transfected with mApple-tauWT or -tauKQ and GFP-EB3. Panels on the right show enlarged images of GFP-EB3 comets within the white boxes. Scale bars, 5 μm. b Movement of an individual GFP-EB3 comet (arrowheads) Scale bar, 2 μm. c, d Quantification of GFP-EB3 movement rate and tau levels in rat primary neurons co-transfected with GFP-EB3 and mApple-tau. n = 11–13 cells/group from two independent experiments. *p < 0.05, one-way ANOVA with Tukey’s post hoc test. Values are mean ± SEM