Fig. 4

K274/281Q tau increases tubulin dynamics in the AIS. a Representative images of a rat primary neuron co-transfected with mApple-tauWT or -tauKQ and GFP-tubulin. White boxes indicate the portion of the AIS (revealed by anti-neurofascin) where GFP-tubulin was photobleached. b Representative images of photobleaching and fluorescence recovery of GFP-tubulin in a ~5-μm portion of the AIS in rat primary neurons transfected with mApple-tauWT or -tauKQ. c Quantification of FRAP of GFP-tubulin in the AIS of rat primary neurons co-transfected with mApple-tauWT or -tauKQ and GFP-tubulin. n = 25–26 cells/group from three independent experiments. **p < 0.01, mixed model linear regression analysis. Values are mean ± SEM. Scale bars, 5 μm