Fig. 5

Stabilization of MTs reduces somatodendritic mislocalization of K274/281Q tau. a–d Photoconversion of mEOS2-tau and its movement in rat primary neurons. a Schematic diagram of photoconversion of mEOS2-tau in the AIS and monitoring its movement toward the somatodendritic compartment and distal axon. b Time-lapse live images of mEOS2-tauWT, -tauKQ, and -tauKR before and after photoconversion in an axon segment ~30 μm from the AIS. The last row represents photoconversion of mEOS2-tauKQ after EpoD treatment (20 nM). White circles indicate the somatodendritic compartment. Scale bars, 10 μm. c, d Quantification of fluorescent intensity in the somatodendritic compartment (c) and distal axon (d) for 30 min after photoconversion of mEOS2-tau in rat primary neurons. n = 8–21 cells/group from three to nine independent experiments. *p < 0.05, **p < 0.01, mixed model linear regression analyses. Values are mean ± SEM