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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Redundant Gs-coupled serotonin receptors regulate amyloid-β metabolism in vivo

Fig. 3

PKA activity modulates production of ISF Aβ. a Selective small molecule inhibitor KT5720 (6 μM), peptide inhibitor PKI14-22 amide (3.6 μM), or vehicle (DMSO or acetonitrile, respectively) were infused via reverse microdialysis (rev md) in 2.5 month old APP/PS1 hemizygous mice for 24 h. In two cohorts of mice after 8 h of inhibitor treatment, the SSRI citalopram was administered by i.p. injection at 10 mg/kg. b Inhibiting PKA with KT5720 alone for 24 h caused a significant increase in ISF Aβx-40 levels by 31.9 ± 6.0 % (p < 0.05; n = 4). By 16 h after co-administration of citalopram with either KT5720 or PKI 14-22, ISF Aβx-40 levels were significantly increased by 45.8 ± 10.5 % (p < 0.05, n = 6) and by 50.6 ± 19.6 % (p < 0.05, n = 6), respectively. Most importantly, each inhibitor completely blocked the effect of citalopram on ISF Aβ levels. Data represented as mean ± SEM. Asterisks mark p-values < 0.05

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