Fig. 5
From: Redundant Gs-coupled serotonin receptors regulate amyloid-β metabolism in vivo
Broad spectrum inhibition of α-secretase enzymes blocks the effects of SSRI. Broad spectrum ADAM/MMP inhibitor GM6001 (25 μM) or vehicle (DMSO) was infused via reverse microdialysis in 2.5 month old APP/PS1 hemizygous mice. 8 h later, the SSRI citalopram was administered by i.p. injection at 10 mg/kg. a As assessed by microdialysis, inhibiting ADAM/MMP enzymes dramatically increased ISF Aβx-40 levels, but there was no reduction in ISF Aβ after SSRI. b After 16 h, vehicle control with SSRI significantly reduced ISF Aβx-40 to 80.9 ± 2.4 % (p = 0.002, n = 6) of levels at hour 8. GM6001 treatment increased ISF Aβx-40 to 264.2 ± 21.1 % at time point 8. After 16 h of SSRI treatment, GM6001 ISF Aβx-40 levels were unchanged at 254.9 ± 16.3 % of baseline (p = 0.74, n = 6). Data represented as mean ± SEM. Asterisks mark p-values < 0.05