Fig. 4

α-synucleinopathy transmission from olfactory structures in older mice. Mice (17 months old) were unilaterally infused in the OB/AON with α-synuclein fibrils (1 h waterbath sonication; see study on older animals in main text and in Additional file 1). Three months later, sagittal brain sections were collected and stained for DRAQ5 (a, c, e, g, i) and pSer129 (b, d, f, h, j). In order to fully appreciate the density of the inclusions and the neuroanatomical sublocalization, the reader must zoom in and out of the stitched pSer129 montages in the high-resolution versions of these files at the link at the end of this article (Additional file 3) or email the corresponding author at leakr@duq.edu for access to the files and examine panels k-m. The extent and density of the label in these fibril-infused animals should be contrasted with the lack of inclusions in large stitched montages of PBS-infused animals in Additional file 1: Figure S8. Abbreviations in Additional file 1: Table S1. k-m Higher magnification views of PBS versus fibril-infused cases. Shown are the olfactory peduncle (k), the hippocampus (l), and the piriform cortex (m). The entire brain section of these PBS cases can be fully examined for background staining in Additional file 1: Figure S8. The scale bar for a-j is in panel A and the scale bar for k-m is in panel m