Fig. 4

Myeloid-specific ikkβ gene deletion decreases the size of lymphatic organs and regulates transcription factors associated with Th1, Th17, and Treg cells. a and b The spleen (a) and lymph nodes (b) were dissected from each group (n = 5) at 15–18 days after immunization and weighted. Bars = 100 mm. c Spinal cords were dissected from each group (n = 5) at 15–18 days after immunization to investigate the degree of recruitment/infiltration of T cells with real-time RT-PCR. The degree of mRNA expression of CD3 was expressed as mean fold-induction ± SEM. d-i Total RNA was isolated from the lumbar spinal cords of each group (n = 3) at days 15–18 post-immunization to measure the level of the mRNA expression of inflammatory mediators with real-time RT-PCR. The mRNA levels of IFN-γ (d), IL-23 (e), IL-17 (f), IL-4 (g), IL-5 (h), and Foxp3 (i) are presented as the fold induction compared with the levels measured in the normal control mice. Data are represented as mean ± SEM. (ANOVA test; **p < 0.01 versus WT EAE mice; #p < 0.05 and #p <0.01 versus normal control mice)