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Table 2 Myeloid-specific ikkβ gene deletion regulates the percentages of CD4+, CD4+/IFN-γ+, CD4+/IL-17+, and CD4+/CD25+/Foxp3+ T cells

From: IKKβ-mediated inflammatory myeloid cell activation exacerbates experimental autoimmune encephalomyelitis by potentiating Th1/Th17 cell activation and compromising blood brain barrier

A
Spinal cord PBS MOG
Wild type LysM-Cre/IkkβF/F Wild type LysM-Cre/IkkβF/F
CD4+ 0.52 ± 0.17 0.65 ± 0.21 14.47 ± 1.21## 8.18 ± 0.53**
CD8+ 2.53 ± 1.38 2.89 ± 0.13 3.07 ± 0.74 2.76 ± 0.06
CD4+/IFN-γ+ 2.97 ± 0.04 2.98 ± 0.95 11.33 ± 0.69## 6.34 ± 0.22**
CD4+/IL-17+ 3.84 ± 0.94 3.01 ± 0.12 14.35 ± 1.21## 5.43 ± 0.63**
CD4+/IL-4+ 0.41 ± 0.09 0.49 ± 0.29 0.87 ± 0.13 0.94 ± 0.27
CD4+/Foxp3+ 0.31 ± 0.45 0.37 ± 0.57 4.59 ± 1.97# 13.49 ± 3.21*
CD4+/CD25+/Foxp3+ 0.46 ± 0.06 0.74 ± 0.10 1.55 ± 0.15## 4.05 ± 0.80*
B     
Lymph node PBS MOG
Wild type LysM-Cre/Ikkβ F/F Wild type LysM-Cre/Ikkβ F/F
CD4+ 8.43 ± 0.64 8.13 ± 0.84 27.77 ± 2.33## 19.96 ± 2.96*
CD8+ 4.85 ± 0.00 4.39 ± 0.10 4.71 ± 1.08 4.06 ± 0.72
CD4+/IFN-γ+ 1.01 ± 0.09 1.24 ± 0.01 9.40 ± 1.98## 4.59 ± 0.72*
CD4+/IL-17+ 0.13 ± 0.53 0.10 ± 0.34 14.76 ± 2.64## 4.13 ± 0.66**
CD4+/IL-4+ 4.55 ± 0.60 4.29 ± 0.52 3.49 ± 1.32## 4.01 ± 0.94
CD4+/Foxp3+ 16.49 ± 1.02 15.21 ± 0.37 8.01 ± 1.31# 11.31 ± 0.20*
CD4+/CD25+/Foxp3+ 5.00 ± 0.21 4.96 ± 0.57 2.75 ± 0.01## 4.41 ± 0.13**
  1. Spinal cords (A) and lymph nodes (B) were dissected from each group (n = 5) at day 15–18 post-immunization to investigate the degree of differentiation and recruitment/infiltration of CD4+ T cells with flow cytometry. Spot plot graphs and their legends in details can be found in the Additional file 4. Data are represented as mean ± SEM. (ANOVA test; * p < 0.05 and **p < 0.01 versus WT EAE mice; #p < 0.05 and ##p <0.01 versus normal control mice)